Defining Suitable Reference Genes for qRT-PCR in Plagiodera versicolora (Coleoptera: Chrysomelidae) under Different Biotic or Abiotic Conditions

Plagiodera versicolora (Coleoptera: Chrysomelidae) is one of the most destructive pests Salicaceae worldwide, which has established complex interactions with surrounding organisms. Uncovering molecular mechanisms some antagonistic would facilitate development environmentally friendly pest insect management strategies. Suitable reference genes are essential for reliable qPCR and gene expression analysis in studies; however, a comprehensive assessment P. still lacking. In this study, stability seven housekeeping (including Actin, EF1A, α-tubulin, RPL13a, RPS18, RPL8 UBC) was investigated under both biotic (developmental stages, tissues, sex pathogen treatment) abiotic (RNA interference treatment, temperature conditions. The geNorm, NormFinder, BestKeeper, ΔCt programs were used to analyze data. RefFinder synthesis applied suggest handful appropriate each experimental condition. RPS18 EF1A different stages; stable female male adults, temperatures, treatment; α-tubulin RPL13a after dietary RNAi treatment. research provides strong basis future into biology versicolora.